Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands
Identifieur interne : 001206 ( Main/Exploration ); précédent : 001205; suivant : 001207Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands
Auteurs : Ben Ma [République populaire de Chine] ; Li-Zhong Liang [République populaire de Chine] ; Gui-Qing Liao [République populaire de Chine] ; Yu-Jie Liang [République populaire de Chine] ; Hai-Chao Liu [République populaire de Chine] ; Guang-Sen Zheng [République populaire de Chine] ; Yu-Xiong Su [République populaire de Chine]Source :
- Journal of Oral Pathology & Medicine [ 0904-2512 ] ; 2013-11.
English descriptors
- Teeft :
- Adenoid, Apoptosis, Assay, Autophagosome, Autophagosome formation, Autophagy, Cancer cells, Cancer therapy, Carcinoma, Cddp, Cell death, Chemical agents, Chemotherapy, Cisplatin, Cisplatin cytotoxicity, Cisplatin treatment, Control group, Cytotoxicity, Different concentrations, Inhibitory, Inhibitory rate, Inhibitory rates, Oncol, Oral pathol, Pathol, Poor sensitivity, Previous studies, Protective role, Protein level, Radiotherapy, Salivary, Salivary gland, Salivary glands, Tumorigenesis, Western blot.
Abstract
Background: The relationship between autophagy and chemotherapy in cancer has been studied a lot recent years. However, there is currently no study on the role of autophagy in chemotherapy of adenoid cystic carcinoma (ACC) of human salivary glands. We hypothesized that autophagy plays a protective role for human salivary gland ACC cells during chemotherapy, diminishes the effect of treatment, and ultimately results in poor sensitivity to chemotherapy. Materials and Methods: After inhibition of autophagy by 5 mM 3‐methyladenine (3MA), 20 μM Chloroquine (CQ), or Beclin‐1 shRNA, we examined the sensitivity of human salivary gland ACC cells to different concentrations of cis‐diamminedichloroplatinum (CDDP) using MTT assay. Also, levels of autophagy in ACC cells treated by CDDP were assessed by western blot, GFP‐LC3 fluorescence and transmission electron microscopy (TEM). Results: Inhibition of autophagy induced by 3MA, CQ, or Beclin‐1 shRNA could all enhance human salivary gland ACC cell death treated by CDDP. And, levels of autophagy in these cells showed a significant increase after treated by CDDP. Conclusion: Autophagy played a protective role for human salivary gland ACC cells during CDDP chemotherapy. Inhibition of autophagy in these cells could enhance cisplatin cytotoxicity‐effects. These findings indicate a novel and promising way to reduce chemotherapy resistance and improve treatment outcome in human salivary gland ACC.
Url:
DOI: 10.1111/jop.12066
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001A09
- to stream Istex, to step Curation: 001A09
- to stream Istex, to step Checkpoint: 000258
- to stream Main, to step Merge: 001207
- to stream Main, to step Curation: 001206
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands</title><author><name sortKey="Ma, Ben" sort="Ma, Ben" uniqKey="Ma B" first="Ben" last="Ma">Ben Ma</name></author><author><name sortKey="Liang, Li Hong" sort="Liang, Li Hong" uniqKey="Liang L" first="Li-Zhong" last="Liang">Li-Zhong Liang</name></author><author><name sortKey="Liao, Gui Ing" sort="Liao, Gui Ing" uniqKey="Liao G" first="Gui-Qing" last="Liao">Gui-Qing Liao</name></author><author><name sortKey="Liang, Yu Ie" sort="Liang, Yu Ie" uniqKey="Liang Y" first="Yu-Jie" last="Liang">Yu-Jie Liang</name></author><author><name sortKey="Liu, Hai Hao" sort="Liu, Hai Hao" uniqKey="Liu H" first="Hai-Chao" last="Liu">Hai-Chao Liu</name></author><author><name sortKey="Zheng, Guang En" sort="Zheng, Guang En" uniqKey="Zheng G" first="Guang-Sen" last="Zheng">Guang-Sen Zheng</name></author><author><name sortKey="Su, Yu Iong" sort="Su, Yu Iong" uniqKey="Su Y" first="Yu-Xiong" last="Su">Yu-Xiong Su</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4756295F731DDF4907B5182AFB4A9B1F9B61E844</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/jop.12066</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-NSBD6NJC-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001A09</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001A09</idno><idno type="wicri:Area/Istex/Curation">001A09</idno><idno type="wicri:Area/Istex/Checkpoint">000258</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000258</idno><idno type="wicri:doubleKey">0904-2512:2013:Ma B:inhibition:of:autophagy</idno><idno type="wicri:Area/Main/Merge">001207</idno><idno type="wicri:Area/Main/Curation">001206</idno><idno type="wicri:Area/Main/Exploration">001206</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands</title><author><name sortKey="Ma, Ben" sort="Ma, Ben" uniqKey="Ma B" first="Ben" last="Ma">Ben Ma</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liang, Li Hong" sort="Liang, Li Hong" uniqKey="Liang L" first="Li-Zhong" last="Liang">Li-Zhong Liang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liao, Gui Ing" sort="Liao, Gui Ing" uniqKey="Liao G" first="Gui-Qing" last="Liao">Gui-Qing Liao</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Liang, Yu Ie" sort="Liang, Yu Ie" uniqKey="Liang Y" first="Yu-Jie" last="Liang">Yu-Jie Liang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liu, Hai Hao" sort="Liu, Hai Hao" uniqKey="Liu H" first="Hai-Chao" last="Liu">Hai-Chao Liu</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zheng, Guang En" sort="Zheng, Guang En" uniqKey="Zheng G" first="Guang-Sen" last="Zheng">Guang-Sen Zheng</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Su, Yu Iong" sort="Su, Yu Iong" uniqKey="Su Y" first="Yu-Xiong" last="Su">Yu-Xiong Su</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guanghua School of Stomatology, Department of Oral and Maxillofacial Surgery, Sun Yat‐Sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Oral Pathology & Medicine</title><title level="j" type="alt">JOURNAL OF ORAL PATHOLOGY AND MEDICINE</title><idno type="ISSN">0904-2512</idno><idno type="eISSN">1600-0714</idno><imprint><biblScope unit="vol">42</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="774">774</biblScope><biblScope unit="page" to="780">780</biblScope><biblScope unit="page-count">7</biblScope><date type="published" when="2013-11">2013-11</date></imprint><idno type="ISSN">0904-2512</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0904-2512</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adenoid</term><term>Apoptosis</term><term>Assay</term><term>Autophagosome</term><term>Autophagosome formation</term><term>Autophagy</term><term>Cancer cells</term><term>Cancer therapy</term><term>Carcinoma</term><term>Cddp</term><term>Cell death</term><term>Chemical agents</term><term>Chemotherapy</term><term>Cisplatin</term><term>Cisplatin cytotoxicity</term><term>Cisplatin treatment</term><term>Control group</term><term>Cytotoxicity</term><term>Different concentrations</term><term>Inhibitory</term><term>Inhibitory rate</term><term>Inhibitory rates</term><term>Oncol</term><term>Oral pathol</term><term>Pathol</term><term>Poor sensitivity</term><term>Previous studies</term><term>Protective role</term><term>Protein level</term><term>Radiotherapy</term><term>Salivary</term><term>Salivary gland</term><term>Salivary glands</term><term>Tumorigenesis</term><term>Western blot</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The relationship between autophagy and chemotherapy in cancer has been studied a lot recent years. However, there is currently no study on the role of autophagy in chemotherapy of adenoid cystic carcinoma (ACC) of human salivary glands. We hypothesized that autophagy plays a protective role for human salivary gland ACC cells during chemotherapy, diminishes the effect of treatment, and ultimately results in poor sensitivity to chemotherapy. Materials and Methods: After inhibition of autophagy by 5 mM 3‐methyladenine (3MA), 20 μM Chloroquine (CQ), or Beclin‐1 shRNA, we examined the sensitivity of human salivary gland ACC cells to different concentrations of cis‐diamminedichloroplatinum (CDDP) using MTT assay. Also, levels of autophagy in ACC cells treated by CDDP were assessed by western blot, GFP‐LC3 fluorescence and transmission electron microscopy (TEM). Results: Inhibition of autophagy induced by 3MA, CQ, or Beclin‐1 shRNA could all enhance human salivary gland ACC cell death treated by CDDP. And, levels of autophagy in these cells showed a significant increase after treated by CDDP. Conclusion: Autophagy played a protective role for human salivary gland ACC cells during CDDP chemotherapy. Inhibition of autophagy in these cells could enhance cisplatin cytotoxicity‐effects. These findings indicate a novel and promising way to reduce chemotherapy resistance and improve treatment outcome in human salivary gland ACC.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Ma, Ben" sort="Ma, Ben" uniqKey="Ma B" first="Ben" last="Ma">Ben Ma</name></region><name sortKey="Liang, Li Hong" sort="Liang, Li Hong" uniqKey="Liang L" first="Li-Zhong" last="Liang">Li-Zhong Liang</name><name sortKey="Liang, Yu Ie" sort="Liang, Yu Ie" uniqKey="Liang Y" first="Yu-Jie" last="Liang">Yu-Jie Liang</name><name sortKey="Liao, Gui Ing" sort="Liao, Gui Ing" uniqKey="Liao G" first="Gui-Qing" last="Liao">Gui-Qing Liao</name><name sortKey="Liu, Hai Hao" sort="Liu, Hai Hao" uniqKey="Liu H" first="Hai-Chao" last="Liu">Hai-Chao Liu</name><name sortKey="Su, Yu Iong" sort="Su, Yu Iong" uniqKey="Su Y" first="Yu-Xiong" last="Su">Yu-Xiong Su</name><name sortKey="Zheng, Guang En" sort="Zheng, Guang En" uniqKey="Zheng G" first="Guang-Sen" last="Zheng">Guang-Sen Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001206 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001206 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:4756295F731DDF4907B5182AFB4A9B1F9B61E844
|texte= Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands
}}
| This area was generated with Dilib version V0.6.33. |  |